Hands-On Life Science

I have used these experiments and demonstrations with elementary school kids--mostly K through 6th grades.

Top of Page ¦ Daphnia Toxicology ¦ Seeds ¦ Taste & Smell

Daphnia Toxicology

Reference:
"Toxicology for Middle School: The Effects of Common Substances on Daphnia", R. N. Russo and S. Parrish, J. Chem. Ed., 72, pp. 49-50, (Jan. 1995).
"A Brine Shrimp Bioassay for Measuring Toxicity and Remediation of Chemicals", Marya Lieberman, J. Chem. Ed., 76, pp. 1689-1691, (Dec. 1999).

Daphnia are small crustaceans commonly found in pond water.
They are fascinating to watch under a microscope. Caffeine caused their heartbeat to increase noticeably, and alcohol depressed it.
You can use Daphnia to test for the toxicity of common substances such as alcohol, tobacco, coffee, and over-the-counter drugs.
Our Daphnia were somewhat finicky creatures. I had to use them as soon as possible after arrival--no later than 24 hours.
See the article for full details.
Be prepared for surprises: we found tobacco to be exceptionally toxic, and alcohol (or at least red wine) definitely prolonged the lifespan of our Daphnia!

If you are doing these experiments at a school, make sure you get permission to bring alcohol, tobacco, and over-the-counter drugs on campus!

Top of Page ¦ Daphnia Toxicology ¦ Seeds ¦ Taste & Smell

Seeds

Examine a variety of seeds:
  • Trees
    • maple ("helicopter")
    • acorn
    • walnut
    • pinecone
  • Fruits & Vegetables
    • apple
    • orange
    • watermelon
    • peach
    • cherry
    • grapes
    • pumpkin
    • corn
    • beans
  • Other Foods
    • sesame seeds
    • poppy seeds
    • caraway seeds
    • sunflower seeds
  • Flowers & Weeds
    • marigold
    • zinnia
    • nasturtium
    • dandelion
    • burrs

Maple seeds and beans are good to dissect.
Try sprouting beans in paper towels in a glass jar.
How do seeds disperse? (Wind; stick to fur and clothes; hidden by squirrels; excreted by birds)
How are seeds protected? (Hard shells, waxy coverings)

Top of Page ¦ Daphnia Toxicology ¦ Seeds ¦ Taste & Smell

Taste & Smell

Reference:
"Why does orange juice taste so bad after you brush your teeth?", Paola DeCristofaro, ChemMatters, April 1995, pp. 14-15.

Taste

Equipment and Materials:

  • salt water (salty)
  • sugar in water (sweet)
  • vinegar (sour)
  • caffeine dissolved in water (strong tea did not work well)
  • small disposable cups
  • Q-Tips

Procedure:
Use a Q-Tip to put a small amount of each liquid (one at a time) on your tongue.
Are some areas of your tongue more sensitive to different tastes?

Smell

Equipment and Materials:
Prepare 20 small vials: in each vial put one of the following substances on a cotton ball or a small piece of filter paper.
Identify each vial with a number.

  • vanilla
  • smoke flavor
  • cinnamon
  • linseed oil
  • orange oil
  • spearmint
  • garlic
  • peppermint
  • root beer
  • vinegar
  • cloves
  • strawberry
  • wintergreen
  • oregano
  • lemon oil
  • anise
  • grape
  • onion
  • basil
  • ammonia

    Procedure:
    See if you can identify the substance from the smell alone.
    This is harder than you think!

    Thanks to Jim Foster at Westvaco Corp. for the donation of a set of "smell bottles".
    Top of Page ¦ Daphnia Toxicology ¦ Seeds ¦ Taste & Smell

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